ABSTRACT
im: Vegetables form a major component of the human diet; however, some agricultural practices may put consumers at risk of parasitic infections. There is high consumption of bitter leaf (Onugbu) in the rural part of Nigeria. They are eaten raw as medicinal herbs by some people. The culture of inadequate or poor washing of vegetables could pose significant and major health threats to the consumers. The aim of the study was to identify some of the parasites that may be attached to the locally consumed edible vegetable Vernonia amygdalina called Onugbu amongst Igbo speaking dialect in south eastern Nigeria. Study Design: This study is a survey that evaluated the parasitic contamination of bitter leaf grown in selected farms in Nimo in Njikoka Local Government Area of Anambra State, Nigeria. Duration: The study lasted for six months. Methodology: V. amygdalina leaf samples were bought directly from the farmers at 300 Naira per rap. The examination of sample was carried out using sedimentation method. Samples weighing 50g were gently washed in normal saline water. The water used in washing was filtered and centrifuged at 300g to concentrate the parasitic stages and also to avoid damages. The sediments obtained were transferred unto labelled clean slides and examined under the light microscope at x 40 objective lens. Results: Parasites were detected in all samples collected from the farm. The commonest parasite was Entamoeba histolytica (cyst 45.5%); other parasites seen are Entamoeba coli (cyst 13.6%), B. coli (trophozoite 27.3%), Ascaris lumbricoides (ova 9.1%) and Strongyloides stercolaris (larva 4. 6%). Conclusion and Recommendations: The results provide evidence of contamination of bitter leaves from farms in Nimo in Njikoka Local Government Area of Anambra State, Nigeria with parasites of public health importance. To avoid infection, humans should wash the leaves of this vegetable with running water or salt and water before it is eating raw.